Nutritionally fastidious Ruminococcus flavefaciens strains

Author: C.E.G. Roché, G.S. Miller & A. Kistner
Year: 1983
Issue: 1
Volume: 13
Page: 57 - 59

The majority of Ruminococcus flavefaciens strains isolated in our laboratories failed to grow or grew poorly both in rumen fluid medium and in a chemically defined medium containing all the nutrients reported to be required for good growth of this species. Attempts were made to develop a defined medium which would support good growth of the R. flavefaciens strains studied. Over 70 different medium formulations free of rumen fluid were tried. However, only one supported growth of all strains and generally growth remained poor. Addition or omission of nutrients, either singly or in combination, from this basal medium, did not result in any material improvement in growth rates.

 

 

Keywords: defined media, nutritional requirements, Ruminococcus albus, Ruminococcus flacefacients, unknown growth factor
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